Human Mesenchymal Stem Cells: Isolation - Culture


Phuc Van Pham
University of Science, Vietnam National University, Ho Chi Minh city, Vietnam


Recent clinical applications of stem cells have demonstrated novel therapies for chronic diseases and certain kinds of cancers. The first clinical case was treated with hematopoietic stem cells over 50 years ago. Subsequently, mesenchymal stem cells (MSCs) have been clinically applied to various diseases. Furthermore, induced pluripotent stem cells were first clinically used in the last year. Some reports suggest that clinical applications of stem cells will increase from 2017 to 2020. One important requirement for the clinical usage of stem cells is the production of stem cells. The quality of stem cells significantly affects the treatment efficiency. This chapter presents some guidelines for MSC production according to good manufacturing practice that helps to maintain the quality of stem cells from batch to batch as well as the clinical satisfaction. Because of the advantages of MSCs, these cells may become the most popular type of stem cells for clinical applications in the near future.


  • Cover page
  • Preface
  • Chapter 1: Mesenchymal stem cells: An introduction
    Page 1-10
  • Chapter 2: Isolation and Culture of Mesenchymal stem cells from bone marrow
  • Chapter 3: Umbilical cord blood derived mesenchymal stem cells: isolation and culture
  • Chapter 4: Isolation of umbilical cord dervied stem cells by tissue expanding culture
  • Chapter 5: Isolation of umbilical cord derived stem cells by single cell culture
  • Chapter 6: Gangival tissue derived stem cells: Isolation and Proliferation
  • Chapter 7: A simple method for adipose tissue derived stem cell culture and proliferation
  • Chapter 8: Menstrual blood derived stem cells: culture and characterization
  • Chapter 9: Isolation of mesenchymal stem cells from peripheral blood
  • Chapter 10: Dental pulp derived stem cells: Isolation and Culture
  • Chapter 11: Placenta derived stem cells: Tissue expanding culture
  • Chapter 12: Isolation and proliferation of Wharton's jelly mesenchymal stem cells
  • Chapter 13: Mesenchymal stem cell suspension culture: micro-carrier culture
  • Conclusion

Author Biography

Phuc Van Pham, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam

Phuc Van Pham received his Ph.D. in Human Physiology from Vietnam National University, Vietnam in 2007. He is currently a Professor of Biology at the University of Science, Vietnam National University in Ho Chi Minh City, Vietnam and Acting Director of the Laboratory of Stem Cell Research and Application. He is a longstanding lecturer and translational scientist at the University, and is a member of several societies and journal editorial boards focused on stem cells.

Dr. Pham and his colleagues have established one of the first multidisciplinary stem cell centers in Vietnam, and he has successfully launched an array of technologies in stem cell isolations. His research interests include stem cell isolation, stem cell therapy, mesenchymal stem cells, cancer stem cells, immunotherapy and regenerative medicine. He has published extensively in these areas.

After many years of experience as an embryologist, cell biologist, and molecular biologist, collaborating with leading researchers in Singapore, Japan, and the United States, Dr. Pham is a student again, keen to reach beyond the traditional boundaries of biology.

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