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Cancer stem cells: Isolation - Culture
The past twenty years have seen a significant worldwide reduction in mortality from cancers, largely due to improved early detection methods and develop- ment of more effective therapies, including adjuvant therapies. However, more than fifty percent of tumors do not respond to these therapies and more than seventy percent of patients relapse after five years. These failures are largely due to cancer stem cells (CSCs), found in all malignant tumors. CSCs are considered to be the origins of tumors and contributors to metastasis and relapse processes in cancer patients. This book summarizes isolation and culture protocols of breast cancer stem cells, liver cancer stem cells, prostate cancer stem cells, leukemia cancer stem cells...